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trypan blue exclusion assay kit  (Millipore)


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    Millipore trypan blue exclusion assay kit
    Trypan Blue Exclusion Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trypan+blue+exclusion+assay+kit/trypan+blue+staining/pm35895804-387-32-37
    Average 90 stars, based on 1 article reviews
    trypan blue exclusion assay kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Trypan Blue Exclusion Assay:

    Article Title: TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.
    Article Snippet: The data was normalized with cell number and analyzed using software Wave (version 2.2.0, Seahorse Bioscience) for visual presence. .. Trypan blue exclusion assay To assess the effect of various transfections or retrovirusbased stable gene expression or treatments on cell proliferation, the cells were trypsinized and number of viable cells counted by trypan blue exclusion assay kit (Sigma, T8154) as per manufacturers protocol and data obtained were represented in bar graphs as mean±SEM. ..

    Transfection:

    Article Title: TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.
    Article Snippet: The data was normalized with cell number and analyzed using software Wave (version 2.2.0, Seahorse Bioscience) for visual presence. .. Trypan blue exclusion assay To assess the effect of various transfections or retrovirusbased stable gene expression or treatments on cell proliferation, the cells were trypsinized and number of viable cells counted by trypan blue exclusion assay kit (Sigma, T8154) as per manufacturers protocol and data obtained were represented in bar graphs as mean±SEM. ..

    Gene Expression:

    Article Title: TNK2/ACK1-mediated phosphorylation of ATP5F1A (ATP synthase F1 subunit alpha) selectively augments survival of prostate cancer while engendering mitochondrial vulnerability.
    Article Snippet: The data was normalized with cell number and analyzed using software Wave (version 2.2.0, Seahorse Bioscience) for visual presence. .. Trypan blue exclusion assay To assess the effect of various transfections or retrovirusbased stable gene expression or treatments on cell proliferation, the cells were trypsinized and number of viable cells counted by trypan blue exclusion assay kit (Sigma, T8154) as per manufacturers protocol and data obtained were represented in bar graphs as mean±SEM. ..



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    Proliferating Jurkat cells were exposed to different doses of avicin D for 24–72 h. A. Cell death was measured by trypan blue exclusion assay. The number of dead cells was counted as a percentage of total cells. B. Cell viability was measured by a CellTiter-Glo Luminescent Cell Viability Assay kit. Data are shown as mean±S.D. (n = 5). The number of surviving cells was counted as a percentage of total cells. C and D. Cell death and cell viability were measured in NB4 cells exposed to 0–4 µg/ml of avicin D for 24–48 h as described in the . Data are shown as mean±S.D. (n = 3). The number of dead or surviving cells was counted as a percentage of total cells. E. Proliferating Jurkat cells were exposed to 2 µg/ml of avicin D for 24 h. Cleavage of Caspase-8 induced by avicin D was analyzed by Western blot. 50 µg of total protein were loaded in each lane. α-Tubulin was detected to serve as an internal control. F. Time-dependent activation of Caspase-8 and apoptotic regulators in avicin D-treated Jurkat and NB4 cells. G. Time-dependent activation of Caspase-8 and apoptotic regulators in avicin D-treated U2OS cells.

    Journal: PLoS ONE

    Article Title: Avicin D, a Plant Triterpenoid, Induces Cell Apoptosis by Recruitment of Fas and Downstream Signaling Molecules into Lipid Rafts

    doi: 10.1371/journal.pone.0008532

    Figure Lengend Snippet: Proliferating Jurkat cells were exposed to different doses of avicin D for 24–72 h. A. Cell death was measured by trypan blue exclusion assay. The number of dead cells was counted as a percentage of total cells. B. Cell viability was measured by a CellTiter-Glo Luminescent Cell Viability Assay kit. Data are shown as mean±S.D. (n = 5). The number of surviving cells was counted as a percentage of total cells. C and D. Cell death and cell viability were measured in NB4 cells exposed to 0–4 µg/ml of avicin D for 24–48 h as described in the . Data are shown as mean±S.D. (n = 3). The number of dead or surviving cells was counted as a percentage of total cells. E. Proliferating Jurkat cells were exposed to 2 µg/ml of avicin D for 24 h. Cleavage of Caspase-8 induced by avicin D was analyzed by Western blot. 50 µg of total protein were loaded in each lane. α-Tubulin was detected to serve as an internal control. F. Time-dependent activation of Caspase-8 and apoptotic regulators in avicin D-treated Jurkat and NB4 cells. G. Time-dependent activation of Caspase-8 and apoptotic regulators in avicin D-treated U2OS cells.

    Article Snippet: Cell death was examined by the cell death enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's protocol of Roche Diagnostics (Pleasanton, CA) or by trypan blue exclusion kit from Sigma-Aldrich (St. Louis, MO) .

    Techniques: Trypan Blue Exclusion Assay, Cell Viability Assay, Western Blot, Activation Assay

    (A & B) Proliferating Jurkat cells were pretreated with 2.5 mg/ml of MCD for 1 h, followed by 2 µg/ml of avicin D for 24 h. A. Cell death was quantified using a cell death ELISA showing enrichment of nucleosomes in the cytoplasmic fraction of Jurkat cells. Values represent the mean±S.D. (n = 3). B. Cell viability was determined by trypan blue exclusion assay. The number of surviving cells was counted as a percentage of total cells. C. MCD inhibits the activity of avicin D in a dose-dependent manner. Increasing concentrations of MCD were applied to Jurkat cells. One hour after treatment with MCD, 2 µg/ml of avicin D were added to the medium. Cell viability was determined by trypan blue exclusion assay after 24 h. Jurkat cells without treatment with MCD and avicin D were set as 100%. D. Jurkat cells were pretreated with 2.5 mg/ml MCD for 1h, followed by 2 µg/ml avicin D for 8 h. The cells were then fixed and stained with FITC-CTxB subunits to identify rafts (green fluorescence) and with anti-Fas antibody to identify Fas (red fluorescence). Area of colocalization between membrane rafts and Fas in the merge panels is yellow.

    Journal: PLoS ONE

    Article Title: Avicin D, a Plant Triterpenoid, Induces Cell Apoptosis by Recruitment of Fas and Downstream Signaling Molecules into Lipid Rafts

    doi: 10.1371/journal.pone.0008532

    Figure Lengend Snippet: (A & B) Proliferating Jurkat cells were pretreated with 2.5 mg/ml of MCD for 1 h, followed by 2 µg/ml of avicin D for 24 h. A. Cell death was quantified using a cell death ELISA showing enrichment of nucleosomes in the cytoplasmic fraction of Jurkat cells. Values represent the mean±S.D. (n = 3). B. Cell viability was determined by trypan blue exclusion assay. The number of surviving cells was counted as a percentage of total cells. C. MCD inhibits the activity of avicin D in a dose-dependent manner. Increasing concentrations of MCD were applied to Jurkat cells. One hour after treatment with MCD, 2 µg/ml of avicin D were added to the medium. Cell viability was determined by trypan blue exclusion assay after 24 h. Jurkat cells without treatment with MCD and avicin D were set as 100%. D. Jurkat cells were pretreated with 2.5 mg/ml MCD for 1h, followed by 2 µg/ml avicin D for 8 h. The cells were then fixed and stained with FITC-CTxB subunits to identify rafts (green fluorescence) and with anti-Fas antibody to identify Fas (red fluorescence). Area of colocalization between membrane rafts and Fas in the merge panels is yellow.

    Article Snippet: Cell death was examined by the cell death enzyme-linked immunosorbent assay (ELISA) according to the manufacturer's protocol of Roche Diagnostics (Pleasanton, CA) or by trypan blue exclusion kit from Sigma-Aldrich (St. Louis, MO) .

    Techniques: Enzyme-linked Immunosorbent Assay, Trypan Blue Exclusion Assay, Activity Assay, Staining, Fluorescence